Characterization of Nucleic Acids

This paper is an analysis conducted on Nucleic Acids through a chassis of stresss particular propositionally, Dische, Murexide, Wheeler-Johnson and Phosphate Tests in vow to exemplify structural features of nucleic acids as well as identify the principle heterogeneous in each chemical rill. Different procedures and different test compounds were applied, and results were notable as for changes in colors of lessens or solutions. For Dische Test, light depressed was obtained for RNA and dark purple for DNA. For Murexide Test, a xanthous-bellied point was obtained with a mixture of red color and eventually became yellow or orange after addition of warm water. For John-Wheeler Test, a violet precipitate was obtained and lastly for Phosphate Test, a yellow precipitate was obtained. Keywords: Nucleic Acids, Dische Test, Murexide Test, Phosphate Test, Wheeler-Johnson Test1. IntroductionThe experiment solely revolved on the appointment of the different structural features tha t make up the coordination compound twist of nucleic acids as well as its characterization through specific chemical tests namely Dische Test, Murexide Test, Wheeler-Johnson Test and Phosphate Test. Upon slaying of the tests mentioned, the principles involved were identified and stated based on the results obtained from the experiment. 2. MethodologyMaterials such as water bathroom, test provides, evaporating dish, test compounds and reagents were gathered and prepared.

In Dische Test, x (10) drops of test compound and Diphenylamine was move in a test tube and heated in boiling water bath for 10 minutes. Color change on the solution was noted. In Murexi! de Test, ten(10) drops of Guanidine was placed in a test tube. Concentrated azotic Acid(HNO3) was added to the test compound and then brought to water bath to run juiceless until a solid mixture is obtained. Once the mixture dry up, ten(10) drops of Potassium Hydroxide(KOH) was used to wet the reagent and then later on put to boiling... If you want to get a full essay, ordain it on our website: BestEssayCheap.com

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